Artificial placenta created in lab

Tinuku - The placenta has an essential and highly complex task: it must ensure the exchange of important substances between the mother and her unborn child, whilst simultaneously blocking other substances from passing through.

Until now, it has not been fully understood what the permeability of the placenta depends on, after all, it is incredibly difficult to investigate its function in humans directly. TU Wien (Vienna) has now produced an artificial placenta model that very closely resembles the natural organ.

Tinuku Artificial placenta created in lab

Using a specially developed femtosecond laser-based 3D printing process, it is possible to produce customized hydrogel membranes directly within microfluidic chips, which are then populated with placenta cells. This means it is now possible to provide clarity in some vital research issues, such as the exchange of glucose between mother and child.

"The transport of substances through biological membranes plays an important role in various areas of medicine. These include the blood-brain barrier, ingestion of food in the stomach and intestine, and also the placenta," says Aleksandr Ovsianikov of the TU Wien.

There are, for example, numerous studies showing that diseases in the mother such as diabetes can have an impact on the unborn child. High blood pressure can also affect the transport of substances to the fetus. Until now, however, it has been almost impossible to investigate the way in which the many parameters involved interact in such cases.

"Our chip consists of two areas, one represents the fetus, the other the mother. We use a special 3D printing process to produce a partition between them, the artificial placenta membrane," Denise Mandt says.

Researchers are therefore working on replicating organ structures on compact chips in order to investigate important aspects of their function under controlled conditions. TU Wien has been working on high-resolution 3D printing of this kind for years, with great success: it involves the use of materials which can be solidified with the help of laser beams.

"In our case it involves a hydrogel with good biocompatibility. Based on the model of the natural placenta, we produce a surface with small, curved villi. The placenta cells can then colonise it, creating a barrier very similar to the natural placenta," Ovsianikov explains.

Tinuku.com Artificial placenta created in lab

This allows the desired 3D structures to be created point by point with a resolution in the micrometre range. On the chip, important biological parameters can be closely monitored, such as the pressure, temperature, geometry and nutrient supply of the mini organs, as well as the administration of medications. This makes it possible to accurately observe disease progression and cure rates.

"This 'organ-on-a-chip' technology is a revolutionary approach in biomedicine, which has generated a great deal of interest in clinical diagnostics, biotechnology and pharmaceutics in recent years," says Peter Ertl.

"The creation of human mini organs on a chip should allow the development of patient-specific therapeutic approaches, and also represents a vital method for replacing animal experiments," Ertl says.

On the chip, important biological parameters can be closely monitored, such as the pressure, temperature, geometry and nutrient supply of the mini organs, as well as the administration of medications. This makes it possible to accurately observe disease progression and cure rates. the team reported to the International Journal of Bioprinting.

Initial tests have already shown that the artificial placenta on the chip does in fact behave in a similar way to a natural placenta: small molecules are allowed to pass through, while large ones are held back. The model is now intended to be used specifically to investigate important aspects of nutrient transport from the mother to the fetus.

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